Western Blot Membrane NC / PVDF

Nitrocellulose:

Supported NitrocelluloseTransfer Membrane combines the binding characteristics of nitrocellulose membrane with the strength of nylon membrane. It can be easily used in any protocol utilizing unsupported nytrocellulose transfer membrane

Product Highlights:

• For procedures that require optimum resolution
• Membrane of choice for protein or immunoblotting applications
• Low background, easily blocked
• BSA binding capacity up to 100μg/cm2
• Wets out naturally
• Compatible with all detection systems

Applications

• Westerns
• Protein & immunoblotting
• Northerns
• Southerns
• Dot/slot blots
• Radiographic, chromogenic and chemiluminescent detection system

PVDF:

PVDF is a naturally hydrophobic, unsupported transfer membrane. It has a high binding capacity, which prevents protein from passing through the membrane, and a low background that provides for an excellent signal-noise ratio. It also has exceptional tensile strength, preventing it from cracking, tearing, breaking or curling.

Product Highlights:

• Can withstand aggressive handling or be used with automated equipment without breaking or tearing
• Ensures tests will be clean with consistent results
• Detects low-level components (exceptional sensitivity)
• Hydrophobic for high protein binding
• BSA protein binding capacity: 125 mg/cm2
• Resistant to most commonly used chemicals compatible with chemically aggressive solvents

Applications

• Western blots
• Immunoblotting
• Solid phase assays
• Amino acid or protein analyses