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FroggaBio Taq DNA Polymerase

FroggaBio Taq DNA Polymerase

NanoTaq Hot-Start DNA Polymerase

NanoTaq Hot-Start DNA Polymerase

Taq FroggaMix Plus

This product can be purchased in the USA exclusively through Gemini-Bio

Click link to buy FBTAQM-PLUS, FBTAQM-PLUS5, FBTAQM-PLUS10

Size:
Grouped product items
Wishlist Product Name Cat# Size Qty
2X Plus FroggaBio Master Mix -1mL
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FBTAQM- PLUS 1 vial
FroggaMix Plus (Taq)
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FBTAQM-PLUS5 1ml X 5 vials
2X Plus FroggaBio Master Mix -10mL
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FBTAQM-PLUS10 1ml X 10 vials

Description

Taq FroggaMix Plus, a mixture of taq and pfu polymerase, blends the processivity of taq with the high fidelity of pfu. Therefore, this specially formulated Taq plus allow amplification of the higer fidelity and longer templates than the single-enzyme formulations. It is also a better choice for amplifying complex template, such as GC-rich template. And it is suitable as a direct replacement for ordinary Taq Polymerase in most applications. In addition, Using Taq plus results in 3´-dA overhangs PCR products, which can be used in TA clone.

Features

High fidelity: Its fidelity is four times higher than ordinary Taq polymerase.
High yields: Suitable for amplifying large fragment, suitable for complex template which is rich in GC or repeat sequence.

Applications
Long PCR with high fidelity
Amplification reaction of complex template

Quality Control 
The absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests

Functionally tested in PCR

Definition of Activity Unit
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.

Storage Buffer 

20mM TrisCl ( pH8.0), 100mM KCl, 3mM MgCl2 1mM DTT,0.1% NP-40 ,0.1% Tween20, 0.2mg/ml BSA, 50% (v/v) glycerol

10X PCR Buffer

100mM Tris-HCl(PH 8.8), 500mMKCl, 1%Triton-X-100, 16mM MgCl2

Store all components at –20°C

Citations

  1. Fernández, D. C., VanLaerhoven, S. L., Rodríguez-Leyva, E., Zhang, Y. M., & Labbé, R. (2022). Population Structure and Genetic Diversity of the Pepper Weevil (Coleoptera: Curculionidae) Using the COI Barcoding Region. Journal of Insect Science22(1), 25.

    Article | FBTAQM-PLUS

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