Taq FroggaMix
Description
2X Taq Mix is a premixed, ready-to-use solution suitable for a wide variety of amplification protocols. It contains everything needed for a standard PCR reaction: Taq DNA Polymerase, dNTPs, Mg2+ and Reaction Buffer. Only primers and template DNA need to be added. Taq DNA Polymerase is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus. The molecular weight is 94 kDa. Taq DNA Polymerase can amplify DNA targets up to 5 kb ( simple template). The elongation velocity is 0.9~1.2kb/min (70~75°C). It has 5' to 3' polymerase activity but lacks 3' to 5' exonuclease activity which results in a 3'-overhang PCR product.
Product Highlights
- Premixed and ready to use
- Includes loading dye
- Saves time and reduces contamination
- High fidelity option offers superior thermostability and proofreading properties (FroggaMix Plus)
Applications
- High-throughput PCR
- Routine PCR with high reproducibility
- Generation of PCR products for TA cloning
- Sequencing
- Experiments requiring high-fidelity (FroggaMix Plus)
Related Products
Protocol
Citations
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Ben Youssef, M., Christelle Ouédraogo, B., Bastarache, P., Dumas, P., Moffat, C. E., Vickruck, J. L., & Morin, P. J. (2022). Exposure to Temperature and Insecticides Modulates the Expression of Small Noncoding RNA-Associated Transcripts in the Colorado Potato Beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae). Journal of Insect Science, 22(1), 23.
Article | FBTAQM
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Pena-Ortiz, M. A., Shafiq, S., Rowland, M. E., & Bérubé, N. G. (2022). Selective isolation of mouse glial nuclei optimized for reliable downstream omics analyses. Journal of Neuroscience Methods, 369, 109480.
Article | FBTAQM
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Pena-Ortiz, M. A., Shafiq, S., Rowland, M. E., & Bérubé, N. G. (2022). Selective isolation of mouse glial nuclei optimized for reliable downstream omics analyses. Journal of Neuroscience Methods, 369, 109480.
Article | FBTAQM
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Kaminker, J. D., & Timoshenko, A. V. (2021). Expression, regulation, and functions of the galectin-16 gene in human cells and tissues. Biomolecules, 11(12), 1909.
Article | FBTAQM
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Simkovich, A. J., Li, Y., Kohalmi, S. E., Griffiths, J. S., & Wang, A. (2021). Molecular Identification of Prune Dwarf Virus (PDV) Infecting Sweet Cherry in Canada and Development of a PDV Full-Length Infectious cDNA Clone. Viruses, 13(10), 2025.
Article | FBTAQM