GenJet Plus DNA In Vitro Transfection Reagent

GenJet™ Plus DNA In Vitro Transfection Reagent is enhanced version of GenJet™ DNA In Vitro Transfection Reagent. GenJet™ Plus is formulated by addition of a booster peptide, giving rise to up to 20 times higher efficiency on variety of mammalian cells in comparison of its previous version. GenJet™ Plus was shown to efficiently deliver genes to various established cell lines as well as primary cells including HEK293, 293T, 293E, CHO, COS1, HeLa, NIH 3T3, insect cell lines (Sf9 and Sf21) and a variety of other eucaryotic cell lines with less toxicity. GenJet™ Plus reagent, 1.0 ml, is sufficient for 667 transfections in 24 well plates or 333 transfections in 6 well plates.

Features compared with old version GenJet™ In Vitro DNA Transfection Reagent
- Cell-dependent 3~20 times higher efficiency
- Top choice for hard-to-transfect cells
- Equally good for very long DNAs (up to 46 kb)
- Efficiency boosted in the presence of serum and antibiotics for most of cell types
- Exceptional high levels of recombinant protein production

Comparisons of Transfection Efficiency of GenJet™ Plus In Vitro DNA Transfection Reagent with the Leading Brand Name Products

GenJet™ Plus is top choice for hard-to-transfect cells. Efficiency comparison of GenJet™ Plus In Vitro DNA Transfection Reagent (left panel) with Lipofectamine 2000 (L2K, right panel) on primary cultured rat aortic smooth muscle cells. A nuclear targeted GFP DNA was transfected with GenJet™ Plus and L2K respectively per manufacturer's protocols. The efficiency was checked 48 hours post transfection. The above pictures were kindly provided by Dr. Nickolai Dulin of Section of Pulmonary and Critical Care, University of Chicago

Left Panel: Comparison of GenJet™ Plus In Vitro DNA Transfection Reagent with leading products including Lipofectamine 2000 (L2K) and Lipofectamine LTX (LTX) on lentivirus production on 293T cells. The data were provided by Dr. Miguel Toscano of Temple University as courtesy.
Right panel: Comparison of GenJet Plus™ In Vitro DNA Transfection Reagent with transfection reagents from other vendors inluding Lipofectamine 2000 (L2K) and FuGene 6 on Hela cells. HeLa cells were transfected with plasmid DNA encoding GFP. Transfections were performed according to manufacturers recommendations and analysed by flow cytometry.

Exceptional high titers of lentivirus generated from 293T cells. Three DNAs co-transfected with GenJet™ Plus In Vitro DNA Transfection Reagent (left panel) in comparison of Lipofectamine LTX (right panel). 1.0 and 10 microliters virus supernatant from 293T cells were used to counter-infected 293F cells which was then passed FACS. The numbers at the upper right corner indicate the percentage of transduced cells. The titers of lentivirus generated with GenJet™ Plus and Lipofectamine LTX were quantified to be 2.1x10⁶ and 9.3x10⁶ tu/ml respectively. The above data were provided by Dr. Miguel Toscano of Temple University as courtesy.