RedSafe™ Nucleic Acid Staining Solution (20,000x) is a new and safe nucleic acid staining solution. It is an alternative to the traditional ethidium bromide(EtBr) stain for detecting nucleic acid in agarose gels. It emits green fluorescence when bound to DNA or RNA. This new stain has two fluorescence excitation maxima when bound to nucleic acid, one centered at 309 nm and another at 419 nm. In addition, it has one visible excitation at 514 nm. The fluorescence emission of RedSafe™ bound to DNA is centered at 537 nm.
RedSafe™ Nucleic Acid Staining Solution (20,000x) is as sensitive as EtBr. The protocol is also similar with a protocol of EtBr. The Ames test proves lower mutation with RedSafe compared with EtBr. In addition, RedSafe™ Nucleic Acid Staining Solution (20,000x) has a negative result in mouse marrow chromophilous erythrocyte micronucleus test and mouse spermary spermatocyte chromosomal aberration test.
RedSafe™ Nucleic acid Staining Solution (20,000x) as compared to EtBr is safer for detecting nucleic acid in agarose gels.
| Product Name: | RedSafe Nucleic Acid Staining Solution | Catalog Number: | 21141 |
| Concentration: | 20,000X | Feature: | Non-toxic, non-mutagenic, non-carcinogenic |
| Excitation Maxima: | 309nm, 419nm | Emission Wavelenght: | 514nm |
| Gel Type: | Nucleic acid gel stain | Target Molecule: | DNA, RNA |
| Ready To Use: | Yes | Detection Method: | Fluorescence |
| Number of Applications: | Sufficient for 200 applications, diluted in 100ml agarose gels | Package: | 1ml x 1vial |
| Storage: | Stable for up to 12 months at room temperature. Stable for up to 24 months at 4℃ | ||
Oghenekaro, A. O., Oviedo-Ludena, M. A., Serajazari, M., Wang, X., Henriquez, M. A., Wenner, N. G., ... & Fernando, W. D. (2021). Population Genetic Structure and Chemotype Diversity of Fusarium graminearum Populations from Wheat in Canada and North Eastern United States. Toxins, 13(3), 180.
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Tian, H., Jin, J., Chen, B., Lefebvre, D. D., Lougheed, S. C., & Wang, Y. (2021). Depth-Dependent Spatiotemporal Dynamics of Overwintering Pelagic Microcystis in a Temperate Water Body. Microorganisms, 9(8), 1718.
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Urai, A., Matsushita, M., Park, H. D., Imachi, H., Ogawara, M., Iwata, H., ... & Takano, Y. (2021). Detection of planktonic coenzyme factor 430 in a freshwater lake: small-scale analysis for probing archaeal methanogenesis. Progress in Earth and Planetary Science, 8(1), 1-17.
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Zimmer, A. M., Mandic, M., Yew, H. M., Kunert, E., Pan, Y. K., Ha, J., ... & Perry, S. F. (2021). Use of a carbonic anhydrase ca17a knockout to investigate mechanisms of ion uptake in zebrafish (Danio rerio). American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 320(1), R55-R68.
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Kelly, J. J., Saee-Marand, M., Nyström, N. N., Evans, M. M., Chen, Y., Martinez, F. M., ... & Ronald, J. A. (2021). Safe harbor-targeted CRISPR-Cas9 homology-independent targeted integration for multimodality reporter gene-based cell tracking. Science advances, 7(4), eabc3791.
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1. DNA
Sensivity of DNA detection of RedSafe Nucleic Acid Staining Solution (20,000x) under UV transmission
Figure1. Gel analysis of serial diluted genomic DNA using RedSafeTM Nucleic Acid Staining Solution (20,000x) and EtBr
2. RNA
Sensivity of RNA detection of RedSafe Nucleic Acid Staining Solution (20,000x) under UV transmission
Figure2. Gel analysis of serial diluted RNA using RedSafeTM Nucleic Acid Staining Solutin (20,000x) and EtBr
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RedSafe gel stain is visible at a wavelength of 514 nm
A: RedSafe stain can be used to detect double-stranded DNA and single-stranded RNA
RedSafe stain shows equivalent sensitivity to EtBr
RedSafe is a non-toxic, non-mutagenic and non-carcinogenic alternative to EtBr. The Ames test proves lower mutation with RedSafe compared with EtBr
RedSafe is room temperature stable for up to 12-months. Prolonged storage can be achieved at 4℃
RedSafe (21141) Safety Data Sheet