RedSafe Nucleic Acid Staining Solution

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Special Price $75.00 $95.00

SKU

21141

Size: 1 mL

Description

Related Products

Specifications

Protocol

Citations

Figures

FAQs

Description

RedSafe™ Nucleic Acid Staining Solution (20,000x) is a new and safe nucleic acid staining solution. It is an alternative to the traditional ethidium bromide(EtBr) stain for detecting nucleic acid in agarose gels. It emits green fluorescence when bound to DNA or RNA. This new stain has two fluorescence excitation maxima when bound to nucleic acid, one centered at 309 nm and another at 419 nm. In addition, it has one visible excitation at 514 nm. The fluorescence emission of RedSafe™ bound to DNA is centered at 537 nm.
RedSafe™ Nucleic Acid Staining Solution (20,000x) is as sensitive as EtBr. The protocol is also similar with a protocol of EtBr. The Ames test proves lower mutation with RedSafe compared with EtBr. In addition, RedSafe™ Nucleic Acid Staining Solution (20,000x) has a negative result in mouse marrow chromophilous erythrocyte micronucleus test and mouse spermary spermatocyte chromosomal aberration test.
RedSafe™ Nucleic acid Staining Solution (20,000x) as compared to EtBr is safer for detecting nucleic acid in agarose gels.

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Specifications

Product Name:	RedSafe Nucleic Acid Staining Solution	Catalog Number:	21141
Concentration:	20,000X	Feature:	Non-toxic, non-mutagenic, non-carcinogenic
Excitation Maxima:	309nm, 419nm	Emission Wavelenght:	514nm
Gel Type:	Nucleic acid gel stain	Target Molecule:	DNA, RNA
Ready To Use:	Yes	Detection Method:	Fluorescence
Number of Applications:	Sufficient for 200 applications, diluted in 100ml agarose gels	Package:	1ml x 1vial
Storage:	Stable for up to 12 months at room temperature. Stable for up to 24 months at 4℃

Protocol

RedSafe Nucleic Acid Staining Solution protocol pdf RedSafe (21141) Safety Data Sheet

RedSafe Nucleic Acid Staining Solution protocol pdf RedSafe (21141) Protocol

Citations

Oghenekaro, A. O., Oviedo-Ludena, M. A., Serajazari, M., Wang, X., Henriquez, M. A., Wenner, N. G., ... & Fernando, W. D. (2021). Population Genetic Structure and Chemotype Diversity of Fusarium graminearum Populations from Wheat in Canada and North Eastern United States. Toxins, 13(3), 180.

Article | 2.5 µL 10× PCR buffer containing MgCl₂ | T-500 | 21141
Tian, H., Jin, J., Chen, B., Lefebvre, D. D., Lougheed, S. C., & Wang, Y. (2021). Depth-Dependent Spatiotemporal Dynamics of Overwintering Pelagic Microcystis in a Temperate Water Body. Microorganisms, 9(8), 1718.

Article | 21141 | 2X Taq Froggamix | SensiFAST SYBR Green Master Mix | 96-well PCR plates
Urai, A., Matsushita, M., Park, H. D., Imachi, H., Ogawara, M., Iwata, H., ... & Takano, Y. (2021). Detection of planktonic coenzyme factor 430 in a freshwater lake: small-scale analysis for probing archaeal methanogenesis. Progress in Earth and Planetary Science, 8(1), 1-17.

Article | 21141
Zimmer, A. M., Mandic, M., Yew, H. M., Kunert, E., Pan, Y. K., Ha, J., ... & Perry, S. F. (2021). Use of a carbonic anhydrase ca17a knockout to investigate mechanisms of ion uptake in zebrafish (Danio rerio). American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 320(1), R55-R68.

Article | 21141
Kelly, J. J., Saee-Marand, M., Nyström, N. N., Evans, M. M., Chen, Y., Martinez, F. M., ... & Ronald, J. A. (2021). Safe harbor-targeted CRISPR-Cas9 homology-independent targeted integration for multimodality reporter gene-based cell tracking. Science advances, 7(4), eabc3791.

Article | 21141

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Figures

1. DNA

Sensivity of DNA detection of RedSafe Nucleic Acid Staining Solution (20,000x) under UV transmission

Figure1. Gel analysis of serial diluted genomic DNA using RedSafeTM Nucleic Acid Staining Solution (20,000x) and EtBr

2. RNA

Sensivity of RNA detection of RedSafe Nucleic Acid Staining Solution (20,000x) under UV transmission

Figure2. Gel analysis of serial diluted RNA using RedSafeTM Nucleic Acid Staining Solutin (20,000x) and EtBr

FAQs

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RedSafe gel stain is visible at a wavelength of 514 nm

A: RedSafe stain can be used to detect double-stranded DNA and single-stranded RNA

RedSafe stain shows equivalent sensitivity to EtBr

RedSafe is a non-toxic, non-mutagenic and non-carcinogenic alternative to EtBr. The Ames test proves lower mutation with RedSafe compared with EtBr

RedSafe is room temperature stable for up to 12-months. Prolonged storage can be achieved at 4℃