PRO-PREP™ Protein Extraction Solution can be simply extract protein from all kinds of cells and tissues. The kit contains 5 kinds of protease inhibitors so it is possible to extract very highly purified proteins. Usually detergent used in protein extraction consists of both hydrophobic tails and amphiphilic molecule and hydrophilic head. The two parts are joined to form a micelle. It is solubilized protein forming a lipid detergent mixed micelle and transmembrane protein forming a protein lipid detergent complex. The extent of micelle formation is termed as CMC(critical micelle concentration). Which is important for high efficiency as well as high purity of protein extraction.
Specifications
Format : Solution type (store at -20c Sample source: Animal cells and tissues Sample size:400ml solution in 5x10(6) cells, 600ml solution in 10-20mg tissues Protein yield:1.9mg-2mg proteins in 5x10(6) cells, 6.5mg-10mg proteins in 10-20mg tissues Prep. time: <30 minutes Included protease inhibitors: PMSF, EDTA, Pepstatin A, Leupeptin, Aprotininl
Protein extraction from cells or tissues
Contains 5 kinds of protease inhibitors (PMSF, EDTA, Pepstatin A, Leupeptin, Aprotinin)
Store at -20c The PRO-PREP(TM) solution doesn't freeze at -20c
Save time (<30 min)
Suitable for Western blotting, Immuno-precipitation (IP), etc.