The Presto™ Midi Plasmid Kit was designed for Plasmid DNA Purification from 50-150 ml of cultured bacterial cells using an efficient plasmid midiprep system. For processing smaller volumes, the Presto™ Mini Plasmid Kit is also available.
Both plasmid kits include TrueBlue Lysis Buffer (an optional color indicator) to prevent common handling errors, while ensuring efficient cell lysis and neutralization. The Presto™ Midi Plasmid Kit uses a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. Using an efficient gravity-flow procedure, plasmid DNA in the crude lysate is bound by the plasmid midi column and the contaminants are removed with PMC Buffer. The purified plasmid DNA is eluted by a high salt buffer and then precipitated with Isopropanol for desalting. The entire procedure can be completed in 80 minutes without ultracentrifuges, HPLC or other toxic reagents.
Advantages
High Yield: 350 µg of pure transfection grade plasmid DNA from 100 ml of cultured bacterial cells
Convenient: TrueBlue Lysis Buffer is an optional color indicator included with this plasmid kit in order to reduce common handlingerrors, while ensuring efficient cell lysis and neutralization and maximum plasmid product yield so you can feel confident with each andevery plasmid prep.
Sample Volume: 50-150 ml of cultured bacterial cells
Format: Plasmid Midi Anion-Exchange Resin Columns and Filter Columns (membrane optimized for plasmid DNA purification)
Elution Volume: 500 µl - 2 ml
Storage:dry at room temperature (15-25°C)
Operation time: within 60 minutes (only 30 minutes when combined with the Presto™ Plasmid Concentrator)
