The Presto™ Max Gel/PCR Kit is a PCR Cleanup Kit and Gel Extraction Kit and in 1 convenient system optimized to recover or concentrate a broad range of DNA fragments (100 bp-50 kb) from agarose gel, PCR, or other enzymatic reactions. For processing 70 bp-20 kb DNA fragments, the Gel/PCR DNA Fragments Extraction Kit is also available. For processing 40-200 bp DNA fragments, the Small DNA Fragments Extraction Kit is also available. The Presto™ Max Gel/PCR Kit uses a concentrated sodium buffer to dissolve agarose gel and denature enzymes. DNA fragments are then bound by silica beads. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer, TE or water. Salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation. Typically, recoveries are up to 85% for Gel Extraction and up to 95% for PCR Cleanup. The entire procedure can be completed in 20 minutes and DNA can be eluted in (10-20 μl) of Elution Buffer to obtain a high concentration of DNA products. The purified DNA is ready for use in PCR, Fluorescent or Radioactive Sequencing, Restriction Enzyme Digestion, DNA Labeling and Ligation.
Advantages (Cat. # DFL100, DFL300)
Fluorescent and Radioactive Sequencing, PCR, Restriction Enzyme Digestion, DNA Labeling and Ligation
Components
Quality Control
The quality of the Presto™ Max Gel/PCR Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. DNA fragments are purified from either agarose gel, PCR products or other aqueous solutions and analyzed by electrophoresis.
Format: Silica Matrix
Binding Capacity: 5 µg
Sample: Agarose Gel Slice/PCR Product
Fragment Size: 100 bp-50 kb
pH Indicator: Yes
Recovery: Up to 95% (<10 kb) Up to 80% (10-50 kb
Elution Volume: 10-20 µl