Nutristem MSC XF Basal Medium

Faster growth rates and stronger cell yields

MSC NutriStem® XF Medium is a defined, serum-free, xeno-free culture medium designed for optimal growth and expansion of human mesenchymal stem/stromal cells (hMSC) derived from a variety of sources, including bone marrow (BM-MSC), adipose tissue (AT-MSC) and umbilical cord matrix (UC-MSC). MSC NutriStem® XF Medium supports long-term growth of hMSC while maintaining their self-renewal and multi-lineage differentiation potential.

hMSCs cultured in MSC NutriStem XF Medium show superior proliferation and self-renewal potential in comparison to serum-containing media and other commercial serum-free media. In addition, hMSCs maintain their proper fibroblast-like cell morphology, tri-lineage differentiation potential, and demonstrate normal hMSC marker profiles, and karyotypic stability over long-term culture.

Key advantages

• Serum-free, xeno-free, defined culture system
• Superior cell growth compared to serum-containing media
• Supports long-term growth and self-renewal of hMSCs from multiple sources
• Consistent, reliable MSC cultures
• Suitable for the harvest of high quality exosomes (EVs) for future clinical applications
• Eliminates the need to spend time and money pre-qualifying FBS lots
• Manufactured under cGMP
• FDA Drug Master File (DMF) available

Cells cultured in serum-containing medium do not require an adaptation phase when transitioning to serum-free MSC NutriStem® XF Medium.

Sample Data

Initial isolation

Figure: Human mesenchymal stem cells were initially isolated from Wharton's jelly (left image) and placenta (right image) using MSC NutriStem XF on pre-coated plates with MSC Attachment Solution (cat. no. 05-752-1). High number of hMSC was obtained.

Proliferation

Figure: MSC NutriStem® XF promotes excellent proliferation of hMSC from a variety of sources while maintaining normal fibroblast-like, spindle shape cell morphology.

Self-renewal potential

Figure: hMSC-BM and hMSC-AT were expanded in MSC NutriStem® XF for 3-5 passages prior to 14 days of CFU-F assay. Colonies were stained with 0.5% crystal violet (x100). The cells maintain their self-renewal potential.

Differentiation potential

Figure: hMSC-BM (top panel) and hMSC-AT (bottom panel) expanded in MSC NutriStem® XF for 3-5 passages prior to trilineage differentiation. Representative images of stained adipocytes (Oil Red O), osteocytes (Alizarin Red) and chondrocytes (Alcian Blue). The cells maintain their multilineage differentiation potential.

Growth curve

Figure: NutriStem® MSC XF Medium promotes superior proliferation and expansion of hMSCs over time as compared to other serum-free and serum-containing media.