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BLUeye Prestained Protein ladder

IRIS11 Prestained Protein Ladder

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This product can be purchased in the USA exclusively through Gemini-Bio Buy Now

Special Price $49.00 $75.00

SKU

PMI11-0500

Size: 500ul

Description

Specifications

Protocol

Figures

FAQs

Description

The IRIS11 Prestained Protein Ladder is a combination of 11 pre-stained proteins with molecular weights from 3 to 260 kDa. The 11 recombinant proteins are covalently coupled with blue chromophore, while 2 red bands at 70 kDa and 260 kDa, a green band at 15 kDa and a newly designed peacock green band at 60 kDa serve as reference bands. The IRIS11 Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating the target protein size and validating the Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.

Specifications

Product Name:	IRIS11 Prestained Protein Ladder	Catalog Number:	PMI11-0500
Molecular Weight Range:	3 to 260 kDa	Reference Bands:	11
Color:	4-color (Blue,Green,Red, Peacock green)	Protein Concentration:	Approximately 0.2~0.4 mg/ml of each protein
Prestained:	Yes	Ready To Use:	Yes
Recommended Gel:	SDS-PAGE (Tris-glycine buffer)	Package:	500μl x 1 vial
Loading Buffer:	20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol	Recommended Loading Volume:	3µl per well (15well) for electrophoresis 5µl per well (10well) for electrophoresis 1.5 - 2.5µl per well for western blotting
Number Of Applications:	Sufficient for 100 application (5μl per loading) Sufficient for 165 application (3μl per loading)	Storage:	Stable for up to 2 weeks at 25°C Stable for up to 3 months at 4°C For long term storage,store at -20°C

Protocol

Iris11 Prestained Protein Ladder SDS pdf Iris11 (PMI11-0500) Safety Data Sheet

Iris11 Prestained Protein Ladder Protocol pdf Iris11 (PMI11-0500) Protocol

Figures

Migration patterns of IRIS11 Prestained Protein Ladder in various electrophoresis conditions:

Refers to iris11 prestained protein ladder patterns in various electrophoresis conditions

Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.

3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively
2.5 μl per well for general Western transferring
Apply more for thicker (> 1.5 mm) or larger gel

All products are for research use only.

More To Explore:

Transfer your target proteins and protein ladder to a Western Blot membrane for detection with our chemiluminescent substrates .

FAQs

Got a question? We're here to answer! If you don't see your question here, drop us a line on our Contact Us page.

This is a four coloured protein ladder

3-5μl per well

PVDF, nylon, and nitrocellulose

No, this protein ladder is ready to use

No, Froggabio protein ladders are ready to load. We do not recommend heating/boiling them as this may cause degradation of proteins in the standard

No, this protein ladder is ready to use

yes, protein marker is another name for protein ladder same as protein molecular weight marker, protein MW marker and protein standards