IRIS11 Prestained Protein Ladder
This product can be purchased in the USA exclusively through Gemini-Bio Buy Now
SKU
PMI11-0500
Description
The IRIS11 Prestained Protein Ladder is a combination of 11 pre-stained proteins with molecular weights from 3 to 260 kDa. The 11 recombinant proteins are covalently coupled with blue chromophore, while 2 red bands at 70 kDa and 260 kDa, a green band at 15 kDa and a newly designed peacock green band at 60 kDa serve as reference bands. The IRIS11 Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating the target protein size and validating the Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.
Specifications
| Product Name: | IRIS11 Prestained Protein Ladder | Catalog Number: | PMI11-0500 |
| Molecular Weight Range: | 3 to 260 kDa | Reference Bands: | 11 |
| Color: | 4-color (Blue,Green,Red, Peacock green) | Protein Concentration: | Approximately 0.2~0.4 mg/ml of each protein |
| Prestained: | Yes | Ready To Use: | Yes |
| Recommended Gel: | SDS-PAGE (Tris-glycine buffer) | Package: | 500μl x 1 vial |
| Loading Buffer: | 20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol |
Recommended Loading Volume: | 3µl per well (15well) for electrophoresis 5µl per well (10well) for electrophoresis 1.5 - 2.5µl per well for western blotting |
| Number Of Applications: | Sufficient for 100 application (5μl per loading) Sufficient for 165 application (3μl per loading) |
Storage: | Stable for up to 2 weeks at 25°C Stable for up to 3 months at 4°C For long term storage,store at -20°C |
Iris11 (PMI11-0500) Safety Data SheetFigures
Migration patterns of IRIS11 Prestained Protein Ladder in various electrophoresis conditions:
Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.
- 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively
- 2.5 μl per well for general Western transferring
- Apply more for thicker (> 1.5 mm) or larger gel
All products are for research use only.
More To Explore:
Transfer your target proteins and protein ladder to a Western Blot membrane for detection with our chemiluminescent substrates .
FAQs
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This is a four coloured protein ladder
3-5μl per well
PVDF, nylon, and nitrocellulose
No, this protein ladder is ready to use
No, Froggabio protein ladders are ready to load. We do not recommend heating/boiling them as this may cause degradation of proteins in the standard
No, this protein ladder is ready to use
yes, protein marker is another name for protein ladder same as protein molecular weight marker, protein MW marker and protein standards