Fast ligation and high transformation efficiency pLUG-Prime® TA-Cloning Vector Kit II
Possible to insert DNA ligation of various sizes in a short time of 5-15 minutes
High true white colony ratio Blue / white colony selection function
Selective culture with ampicillin resistant gene
Use M13 primer site for convenient sequencing
Application of highly restrictive enzymes
EcoRI restriction enzyme site is on both sides of MCS (Multi cloning site), enabling enzyme cutting at one time
pLUG-Prime® TA-cloning Vector Kit II features fast ligation and high transformation efficiency. Especially, it is designed to ligation within 5-15 minutes. It shows high true white colony ratio in various size insert DNA. It is also possible to cultivate selectively using ampicillin resistant gene. Blue / White colony sorting function makes it easier to select target transformants. The pLUG-Prime® TA-cloning Vector Kit II TA-cloning site is more common and is designed to have a well- known restriction enzyme site on which, in particular, its predecessor pLUG-Prime® TA-cloning EcoRI restriction enzyme site relative to both Vector Kit This method makes it easy to carry out restriction analysis of recombinant plasmids and re-cloning into other vectors.