The Genomic DNA Mini Kit (Plant) is a genomic DNA extraction kit optimized for genomic, mitochondrial and chloroplast DNA purification from plant tissue. This DNA extraction kit processes plant samples by grinding in liquid nitrogen followed by lysis buffer incubation. The lysate is treated with RNase A then filtered to remove cell debris and salt precipitates. In the presence of the binding buffer, coupled with chaotropic salt, genomic DNA in the lysate binds to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. DNA phenol extraction or alcohol precipitation is not required, and the entire procedure can be completed in less than 30 minutes. For RNA purification from plant samples, the Total RNA Mini Kit (Plant) is also available.
Applications
PCR, Real-time PCR, Southern Blotting and RFLP
Components
Quality Control
The quality of the Genomic DNA Mini Kit (Plant) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Genomic DNA is extracted from 50 mg fresh young leaf samples. The purified genomic DNA (>10 µg) is quantified with a spectrophotometer and checked by electrophoresis.
Format: Spin Column
Binding Capacity: 50 µg (Mini) 500 µg (Maxi)
Sample: Mini: 100 mg plant tissue, Maxi: 1 g plant tissue
Micropestle: N/A
Filter Column: Yes
Enzyme: N/A
Typical Yield: 3-5 µg from 100 mg Arabidopsisleaf (Mini),80 µg from 500 mg Lemon leaf (Maxi)
