GENEzol™ TriRNA Bacteria Kit is a phenol, chloroform and guanidine isothiocyanate based scalable solution for extracting high-quality total RNA as well as simultaneous RNA, DNA and protein extraction from bacteria samples. The provided Lysozyme and Bacteria Lysis Buffer will efficiently lyse bacterial cell walls consisting of the peptidoglycan layer. The extracted RNA can be used directly in a variety of downstream applications.
Advantages (Cat. # GZB050, GZB100)
Extract total RNA or simultaneous RNA, DNA and protein within 1 hour
Storage: GENEzol™ Reagent should be stored at 2ºC to 25ºC for up to 9 months, Bacteria Lysis Buffer should be stored at room temperature (15-25ºC) for up to 9 months, Lysozyme should be stored at -20ºC for extended periods
GENEzol™ TriRNA Bacteria Kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. AnEscherichia coli (1 × 109) culture (OD600=1.3, 1 ml) is harvested by centrifugation at 16,000 x g for 2 minutes, followed by RNA extraction. 10 µl from a 50 µl eluate of RNA is analyzed by electrophoresis on a 0.8% agarose gel.
