Total RNA Mini Kit (Tissue) - 50preps/kit

The Total RNA Mini Kit (Tissue) is a RNA Isolation Kit designed for total RNA purification from a variety of animal tissue and paraffin-embedded tissue using an efficient RNA miniprep system. This RNA Kit will purify RNA from tissue samples within 15 minutes. Tissue samples can be efficiently homogenized in a microcentrifuge tube using the provided DNase and RNase-free Micropestle. TheTotal RNA Mini Kit (Tissue) uses detergents and chaotropic salt to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound by the glass fiber matrix of the RNA spin column. Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free Water. Phenol extraction or alcohol precipitation is not required and the purified RNA is ready for use in a variety of downstream applications. Each Geneaid RNA Kit includes individually packaged certified DNase and RNase-free spin columns and collection tubes.

 Advantages (Cat. # RT050, RT100, RT300)

• Purify total RNA within 15 minutes!
• Sample: up to 25 mg of tissue, up to 25 mg of paraffin-embedded tissue
• High Yield: 5-30 μg of pure RNA
• RNase and DNase-free Micropestles included for simple tissue homogenization within the tube to reduce the risk of sample contamination
• Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free
• RNA glass fiber spin columns (yellow membrane optimized for total RNA extraction)
• Storage: dry at room temperature (15-25ºC) for up to 9 months

Applications

 

RT-PCR, Northern Blotting, Primer Extension, mRNA Selection, cDNA Synthesis, RNase Protection Assay

 

Components

• Micropestles
• RB Buffer
• W1 Buffer
• Wash Buffer
• RNase-free Water
• RB Columns
• Filter Columns
• 2 ml Collection Tubes

 Quality Control

 The quality of the Total RNA Mini Kit (Tissue) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Total RNA from a 25 mg fresh tissue sample is extracted using the Total RNA Mini Kit (Tissue). The purified RNA (A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.