Presto Mini RNA Bacteria Kit

The Presto™ Mini RNA Bacteria Kit was designed for total RNA purification from Gram (-) negative bacteria and Gram (+) positive bacteria using an efficient RNA miniprep system. This RNA Kit includesBacteria Lysis Buffer and Lysozyme to reduce sample preparation time and minimize hands on time.  ThePresto™ Mini RNA Bacteria Kit uses detergents and chaotropic salt to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound by the glass fiber matrix of the RNA spin column and once any contaminants have been removed, using the Wash Buffer, the purified total RNA is eluted by RNase-free Water. High quality total RNA can be purified within 20 minutes and is ready for use in RT-PCR, Northern Blotting, Primer Extension, mRNA Selection and cDNA Synthesis.  Each Geneaid RNA Kit includes individually packaged certified DNase and RNase-free spin columns and collection tubes.

Advantages (Cat. # RBB050, RBB100, RBB300)

• Purify total RNA within 20 minutes!
• High Yield: up to 60 μg of pure RNA (1 x 109 Escherichia coli: 40-45 µg, 1 x 109 Bacillus subtilis: 50-55 µg)
• Wide Variety of Bacteria Sample Strains: Gram (+) Positive and Gram (-) Negative bacterial cells (up to 1 x 109)
• Convenient: Includes Bacteria Lysis Buffer and Lysozyme
• RNA glass fiber spin column (yellow membrane optimized for total RNA purification from bacteria)
• Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free
• Storage: dry at room temperature (15-25°C) for up to 9 months, Lysozyme should be stored at -20°C for extended periods

• Bacteria Lysis Buffer
• Lysozyme
• RB Buffer
• W1 Buffer
• Wash Buffer
• RNase-free Water
• RB Columns
• 2 ml Collection Tubes

Quality Control

The quality of the Presto™ Mini RNA  Bacteria Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. The purified RNA from Escherichia coli cells (up to 1 x 109), (A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.