Presto Endotoxin Free Mini Plasmid Kit

The Presto™ Endotoxin Free Mini Plasmid Kit was designed for transfection grade plasmid DNApurification from 1-5 ml of cultured E. coli cells using silica-membrane spin columns. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. In addition, an efficient endotoxin removal step is integrated in the procedure and endotoxins are precipitated in a proprietary reagent. TrueBlue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 50 minutes. The purified endotoxin free plasmid DNA is ready for use in transfection, restriction enzyme digestion, ligation, PCR, and sequencing reactions.
 

• Endotoxin Free, Transfection Grade Plasmid DNA: <0.1 EU/µg of plasmid DNA
• Convenient: TrueBlue Lysis Buffer is an optional color indicator included with the kit to reduce common handling errors, while ensuring efficient cell lysis, neutralization and maximum plasmid product yield so you can feel confident with each and every plasmid prep.
• Plasmid Size: 1-15 kb
• High Yield: up to 40 µg of transfection grade plasmid DNA
• Sample Volume: 1-5 ml of cultured bacterial cells
• Elution Volume: 30-100 µl
• Spin Columns: glass fiber membrane optimized for plasmid DNA purification
• Storage: room temperature (15-25°C) for up to 1 year without showing any reduction in performance

 Applications

 Transfection, Restriction Enzyme Digestion, Library Screening, Ligation, PCR, Transformation and Sequencing Reactions

Components

• PE1, PE2, PE3, PE4, PE5 Buffers
• TrueBlue Lysis Buffer
• W1 Buffer
• Wash Buffer
• Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
• RNase A
• PDH Columns
• 2 ml Collection Tubes

 Quality Control

 The quality of the Presto™ Endotoxin Free Mini Plasmid Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Plasmid DNA from a 4 ml overnight E. coli (DH5α) culture, containing plasmid pBluescript (A600 > 2 U/ml) is purified using the Presto™ Endotoxin Free Mini Plasmid Kit. Following the purification process, a yield of more than 25 μg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.