The Geneius™ Micro gDNA Extraction Kit provides an efficient method for purifying DNA (including genomic and mitochondrial DNA) from small volumes of whole blood, dried blood spots and urine. This DNA Extraction Kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. S3 Powder is included with this DNA extraction kit to improve the efficiency of DNA binding to the spin column membrane. Contaminants are removed using W1 Buffer and Wash Buffer (containing ethanol). The purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed in as little as 20 minutes without phenol/chloroform extraction or alcohol precipitation, with an average DNA yield of 300 ng from 6 mm dried blood spots. Purified DNA, with approximately 20-30 kb, is suitable for use in PCR or other enzymatic reactions.
Advantages
Purify genomic DNA from small volume blood samples, dried blood spots and urine
Time: in as little as 20 minutes
High Yield: 300 ng of pure genomic DNA from 6 mm blood spots
High Purity gDNA: A260/A280 = 1.8-2.0
DNA Size: 20-30 kb
Genomic DNA glass fiber spin column (green membrane optimized for genomic DNA purification from small volume samples)
Elution volume: 50-100 µl
Storage: dry at room temperature (15-25°C) for up to 1 year
Applications
PCR, Restriction Endonuclease Digestions, Array, Southern Blots and various other enzymatic reactions
Components
S1 Buffer
S2 Buffer
S3 Powder
W1 Buffer
Wash Buffer
Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
Proteinase K
GD Columns
2 ml Collection Tubes
Quality Control
The quality of the Geneius™ Micro gDNA Extraction Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. The purified genomic DNA (300 ng with an A260/A280 ratio of 1.8-2.0) from 10 µl blood samples is quantified with a spectrophotometer and checked by electrophoresis.
