Presto DNA/RNA Extraction Kit, 50 preps/kit

• Purify genomic DNA and total RNA within 25 minutes!
• Variety of Samples: up to 500 μl of whole blood, up to 25 mg of tissue, rodent tails, ear punches, up to 5 x 10⁶ cultured cells, up to 500 μl of biological fluids
• High Yield: up to 3 μg of genomic DNA and up to 15 μg of total RNA from 1 x 10⁶ of cultured HeLa cells
• High Quality DNA: A260/A280 = 1.9-2.0
• Genomic DNA glass fiber spin columns and total RNA glass fiber spin colums certified DNase and RNase-free
• Elution Volume: 50-200 μl (genomic DNA) and 25-50 μl (total RNA)
• Storage: dry at room temperature (15-25°C) for up to 9 months

Applications

The purified DNA, approximately 20-30 kb, is suitable for use in PCR or other enzymatic reactions and the purified RNA (including miRNA) is ready for use in RT-PCR, Real-time PCR, northern blotting, primer extension, mRNA selection and cDNA synthesis.

Components

• RBC Lysis Buffer
• DR Buffer
• RW1 Buffer
• RPE Buffer
• W1 Buffer
• Wash Buffer
• RNase-free Water
• Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
• GD Columns
• RB Columns
• 2 ml Collection Tubes

Quality Control

The quality of the Presto™ DNA RNA Extraction Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system by isolating genomic DNA and total RNA from 1 x 10⁶ cultured animal cells. The purified DNA and total RNA is quantified with a spectrophotometer and analyzed by electrophoresis on a 1% agarose gel.