The Presto™ 96 Well Blood Genomic DNA Extraction Kit was designed for high-throughput purification of genomic, mitochondrial and virus DNA from whole blood (fresh blood and frozen blood), plasma, serum, body fluids, lymphocytes and cultured cells. This DNA extraction kit uses protease and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the Presto™ gDNA 96 Well Binding Plate. Contaminants are removed using a Wash Buffer and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The procedure can be completed within 45 minutes without phenol/chloroform extraction or alcohol precipitation. The purified genomic DNA (approximately 20-30 kb) is suitable for use in PCR or other enzymatic reactions
Advantages (Cat. # 96GBP04, 96GBP10)
Purify 96 wells of pure genomic DNA in 45 minutes!
Sample Volume: up to 200 µl of whole blood, plasma, serum, body fluids and up to 5 x 106 lymphocytes or cultured cells per well
Yield: up to 6 μg of genomic DNA from 200 µl of whole blood per well
Format: Presto™ 96 Well gDNA Binding Plates
Centrifuge or vacuum manifold protocol
Storage: dry at room temperature (15-25ºC) for at least 1 year without showing any reduction in performance
The quality of the Presto™ 96 Well Blood Genomic DNA Extraction Kit is tested on a lot-to-lot basis by purifying genomic DNA from 200 μl of whole blood samples. The purified DNA is quantified with a spectrophotometer and analyzed by electrophoresis.
