Presto 96 Well Blood Genomic DNA Extraction Kit

The Presto™ 96 Well Blood Genomic DNA Extraction Kit was designed for high-throughput purification of genomic, mitochondrial and virus DNA from whole blood (fresh blood and frozen blood), plasma, serum, body fluids, lymphocytes and cultured cells. This DNA extraction kit uses protease and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the Presto™ gDNA 96 Well Binding Plate. Contaminants are removed using a Wash Buffer and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The procedure can be completed within 45 minutes without phenol/chloroform extraction or alcohol precipitation. The purified genomic DNA (approximately 20-30 kb) is suitable for use in PCR or other enzymatic reactions

 Advantages (Cat. # 96GBP04, 96GBP10)

• Purify 96 wells of pure genomic DNA in 45 minutes!
• Sample Volume: up to 200 µl of whole blood, plasma, serum, body fluids and up to 5 x 10⁶ lymphocytes or cultured cells per well
• Yield: up to 6 μg of genomic DNA from 200 µl of whole blood per well 
• Format: Presto™ 96 Well gDNA Binding Plates
• Centrifuge or vacuum manifold protocol
• Storage: dry at room temperature (15-25ºC) for at least 1 year without showing any reduction in performance

Applications

PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR

Components

• GB Buffer
• Protease
• W1 Buffer
• Wash Buffer
• Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
• Presto™ gDNA 96 Well Binding Plates
• 96 Deep Well Plates
• Microtubes (Racked)
• Microtubes (8-strip)
• Caps for Microtubes (8-strip)

Quality Control

The quality of the Presto™ 96 Well Blood Genomic DNA Extraction Kit is tested on a lot-to-lot basis by purifying genomic DNA from 200 μl of whole blood samples. The purified DNA is quantified with a spectrophotometer and analyzed by electrophoresis.