Presto™ 96 Well PCR Cleanup Kits were designed to recover, concentrate or purifify DNA fragments from PCR or other enzymatic reactions using an efficient 96 well binding plate system. This PCR Purification Kit uses chaotropic salt denature enzymes allowing DNA fragments to be bound by the glass fiber matrix in each well of the 96 well binding plate. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer, TE or water. Salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation and the purified DNA is ready for use in subsequent reactions. ThePresto™ 96 Well PCR Cleanup Kit is a cost effective, high performance alternative for efficient DNA fragments PCR purification.
Advantages (Cat. # 96DFH04, 96DFH10)
Applications
PCR, Fluorescent or Radioactive Sequencing, Restriction Enzyme Digestion, DNA Labeling and Ligation
Components
Quality Control
The quality of the Presto™ 96 Well PCR Cleanup Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. DNA fragments of various sizes are purified from PCR products and analyzed by electrophoresis.
Format: 96 Well Plate
Binding Capacity: 10 µg
Sample: PCR Product
Fragment Size: 70 bp-20 kb
pH Indicator: N/A
Recovery: Up to 95%
Elution Volume: 60-80 µl
Operation Time: 20 minutes
