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Presto 96 Well PCR Cleanup Kit

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$1,066.00

SKU

96DFH04

Size: 4 x 96 well plates/kit

Description

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Specifications

Description

Presto™ 96 Well PCR Cleanup Kits were designed to recover, concentrate or purifify DNA fragments from PCR or other enzymatic reactions using an efficient 96 well binding plate system. This PCR Purification Kit uses chaotropic salt denature enzymes allowing DNA fragments to be bound by the glass fiber matrix in each well of the 96 well binding plate. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer, TE or water. Salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation and the purified DNA is ready for use in subsequent reactions. ThePresto™ 96 Well PCR Cleanup Kit is a cost effective, high performance alternative for efficient DNA fragments PCR purification.

Advantages (Cat. # 96DFH04, 96DFH10)

Purify up to 100 µl of PCR products per well in 20 minutes!
High Recovery: up to 95%
Format: Presto™ PCR Cleanup 96 Well Binding Plates
Broad Fragment Size Range: 70 bp–20 kb
Centrifuge or vacuum manifold protocol
Elution Volume: 60 µl from 80 µl elution buffer volume, 40 µl from 60 µl elution buffer volume
Storage: dry at room temperature (15-25ºC) for up to 1 year

Applications

PCR, Fluorescent or Radioactive Sequencing, Restriction Enzyme Digestion, DNA Labeling and Ligation

Components

PB Binding Buffer
W1 Buffer
Wash Buffer
Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
Presto™ PCR Cleanup 96 Well Binding Plates
0.35 ml Collection Plates
Adhesive Film

Quality Control

The quality of the Presto™ 96 Well PCR Cleanup Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. DNA fragments of various sizes are purified from PCR products and analyzed by electrophoresis.

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Specifications

Format: 96 Well Plate

Binding Capacity: 10 µg

Sample: PCR Product

Fragment Size: 70 bp-20 kb

pH Indicator: N/A

Recovery: Up to 95%

Elution Volume: 60-80 µl

Operation Time: 20 minutes