GENEzol TriRNA Pure Kit

The GENEzol™ TriRNA Pure Kit is a phenol and guanidine isothiocyanate plus spin column system for convenient purification of high-quality total RNA from a variety of samples. Initially, samples are homogenized in GENEzol™ Reagent without chloroform phase separation or isopropanol RNA precipitation. Following sample homogenization, simply bind, wash and elute the high-quality, total RNA in RNase-free Water and use in a variety of sensitive downstream applications.

Advantages (Cat. # GZXD050, GZXD100, GZXD200)

• Purify total RNA in 15 minutes!
• A cost effective phenol, guanidine isothiocyanate solution plus spin column system
• No chlorophorm phase separation
• No isopropanol RNA precipitation
• No phenol carryover
• Sample Volume: up to 200 μl of blood, buffy coat, serum, plasma, up to 5 x 106 cultured cells, 10-50 mg of tissue, 1 x 109bacterial cells, 20-50 mg of plant tissue
• High quality RNA: A260/A280 >1.8, A260/A230 >1.8
• Binding Capacity: 50 µg RNA from ≥ 25 µl DNase/RNase-free Water/li>
• Spin Columns: glass fiber membrane optimized for total RNA extraction (certified RNase and DNase-free)
• Storage: GENEzol™ Reagent should be stored at 2ºC to 25ºC for up to 9 months, DNase should be stored at -20ºC for up to 9 months, other components should be stored at room temperature (15-25ºC) for up to 9 months

Applications

cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays, Northern Blotting

Components

• GENEzol™ Reagent
• Pre-Wash Buffer
• DNase I
• DNase I Reaction Buffer
• Wash Buffer
• RNase-free Water
• RB Columns
• 2 ml Collection Tubes

Quality Control

The GENEzol™ TriRNA Pure Kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system.