Antibody Stripping Buffer

Western Blot is widely used to detect and compare proteins from a complex mixture utilizing antibody detection on a membrane. Chemiluminescence has become an easy and sensitive method of detection compared to other analysis. Because of the nature of chemiluminescence detection, it is possible to reprobe the separated protein mixture on the membrane. Conventionally, Western blots have been stripped using extremely harsh conditions that may alter the antigen for subsequent immunoprobing. Gene Bio-Application, Stripping buffer is a novel formula provides a gentle method of removing primary and secondary antibodies membranes that allows several reprobings on the same membrane.