2X FroggaBio Master Mix -10mL

Description

2X Taq Mix is a premixed, ready-to-use solution containing Taq DNA Polymerase, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR, as well as an inert loading dye. To prepare the final PCR, only primers and template DNA need to be added. This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set up. The mix retains all features of Taq DNA Polymerase. Taq DNA Polymerase is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus. The molecular weight is 94 kDa. Taq DNA Polymerase can amplify DNA targets up to 5 kb ( simple template). The elongation velocity is 0.9~1.2kb/min (70~75°C). It has 5' to 3' polymerase activity but lacks 3' to 5' exonuclease activity which results in a 3'-dA overhang PCR product.

Applications

• High-throughput PCR.
• Routine PCR with high reproducibility
• Generation of PCR products for TA cloning

Contents:

• 2X Taq Mix 1ml
• Nuclease-free water 1ml