FroggaBio Taq DNA Polymerase
Description
Taq DNA Polymerase is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus. The molecular weight is 94 kDa. Taq DNA Polymerase can amplify DNA targets up to 5 kb ( simple template). The elongation velocity is 0.9~1.2kb/min (70~75°C). It has 5' to 3' polymerase activity but lacks 3' to 5' exonuclease activity which results in a 3'- overhang PCR product.
Includes everything needed for a standard PCR reaction:
- Vial 1-Taq DNA Polymerase
- Vial 2-10xPCR Buffer (Mg 2+ Plus)
- Vial 3-dNTPs
- Vial 4-6 x Loading Buffer
Once your master mix is made, only primers and template DNA need to be added.
Product Highlights
- Includes loading dye
- Great for standard PCR applications
Applications
- High-throughput PCR
- Routine PCR with high reproducibility
- Generation of PCR products for TA cloning
- Sequencing
Related Products
Protocol
Citations
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Oghenekaro, A. O., Oviedo-Ludena, M. A., Serajazari, M., Wang, X., Henriquez, M. A., Wenner, N. G., ... & Fernando, W. D. (2021). Population Genetic Structure and Chemotype Diversity of Fusarium graminearum Populations from Wheat in Canada and North Eastern United States. Toxins, 13(3), 180.
Article | 2.5 µL 10× PCR buffer containing MgCl2 | T-500 | 21141
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Morrison, Z. A., Eddenden, A., Subramanian, A. S., Howell, P. L., & Nitz, M. (2022). Termination of Poly-N-acetylglucosamine (PNAG) Polymerization with N-Acetylglucosamine Analogues. ACS Chemical Biology.
Article | T-500
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Gagnon, M., Hamelin, L., Fréchette, A., Dufour, S., & Roy, D. (2020). Effect of recycled manure solids as bedding on bulk tank milk and implications for cheese microbiological quality. Journal of dairy science, 103(1), 128-140.
Article | Geneaid Presto Mini gDNA Bacteria Kit | 21141 | DM003-R500 | T-500
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Doering, J. A., Booth, T., Wiersma, Y. F., & Piercey-Normore, M. D. (2020). How do genes flow? Identifying potential dispersal mode for the semi-aquatic lichen Dermatocarpon luridum using spatial modelling and photobiont markers. BMC ecology, 20(1), 1-15.
Article | 1x PCR buffer | T-500 | dNTP
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Williams, A. N., & Stavrinides, J. (2020). Pantoea natural product 3 is encoded by an eight-gene biosynthetic gene cluster and exhibits antimicrobial activity against multi-drug resistant Acinetobacter baumannii and Pseudomonas aeruginosa. Microbiological research, 234, 126412.
Article | T-500