Need exosomes? SBI’s ExoQuick-TC enables high-throughput, quantitative isolation of exosomes from low volumes (as little as 1 ml) of tissue culture media and certain biofluids (saliva, urine, follicular fluid, and breast milk). Note, to isolate exosomes from serum, plasma, or ascites fluid, use the original ExoQuick formulation (EXOQ5A-1 or EXOQ20A-1)). Compatible with a wide variety of downstream applications, ExoQuick-TC is an effective and proven alternative to ultracentrifugation1-3.
ExoQuick-TC’s fast, ultracentrifugation-free method:
ExoQuick-TC is a proprietary polymer that gently precipitates exosomes. First, pre-clear your samples of cells and cellular debris, and then simply add the appropriate amount of ExoQuick-TC to your cleared biofluid, refrigerate, and centrifuge (see the product manual for protocol details). Your exosomes will be in the pellet, ready for resuspension in an appropriate solution.
How It Works: High-throughput, quantitative exosome recovery
ExoQuick-TC can be used to purify exosomes from a wide variety of tissue culture media4, and from certain biofluids such as saliva1, urine2, follicular fluid3, and breast milk5. With a simple workflow involving minimal hands-on time and low input sample volume requirements, ExoQuick-TC is an excellent option for researchers who need to purify multiple exosome samples.
To isolate exosomes from tissue culture media, simply:
Isolated exosomes can be found in the pellet and resuspended in an appropriate solution.
With ExoQuick-TC, you can obtain high-quality exosomes from tissue culture media and certain biofluids using a protocol that can easily be performed on multiple samples and requires very low volumes of input sample