ExoQuick TC 10ml

ExoQuick TC 10ml

DNS300 RNase-Free DNase I Set

RNase-Free DNase I Set

ExoQuick TC 50ml

ExoQuick TC 50ml

ExoQuick TC 10ml

Fast, scalable exosome isolation from tissue culture media
$341.00
SKU
EXOTC10A-1
Size: 10 ml

Description

 Need exosomes? SBI’s ExoQuick-TC enables high-throughput, quantitative isolation of exosomes from low volumes (as little as 1 ml) of tissue culture media and certain biofluids (saliva, urine, follicular fluid, and breast milk). Note, to isolate exosomes from serum, plasma, or ascites fluid, use the original ExoQuick formulation (EXOQ5A-1 or EXOQ20A-1)). Compatible with a wide variety of downstream applications, ExoQuick-TC is an effective and proven alternative to ultracentrifugation1-3.

ExoQuick-TC’s fast, ultracentrifugation-free method:

  • Saves time and labor
  • Is easily scalable
  • Conserves precious sample
  • Delivers high yields of functional, high quality exosomes
  • Can be used to isolate exosomes for a wide range of downstream applications, including
    • Biomarker studies
    • Exosomal miRNA profiling
    • Exosomal proteomics
    • Exosomal lipidomics/metabolomics
    • Functional studies, such as in cell-to-cell signaling
    • Basic biology, such as role in tumorigenesis

ExoQuick-TC is a proprietary polymer that gently precipitates exosomes. First, pre-clear your samples of cells and cellular debris, and then simply add the appropriate amount of ExoQuick-TC to your cleared biofluid, refrigerate, and centrifuge (see the product manual for protocol details). Your exosomes will be in the pellet, ready for resuspension in an appropriate solution.

How It Works: High-throughput, quantitative exosome recovery

ExoQuick-TC can be used to purify exosomes from a wide variety of tissue culture media4, and from certain biofluids such as saliva1, urine2, follicular fluid3, and breast milk5. With a simple workflow involving minimal hands-on time and low input sample volume requirements, ExoQuick-TC is an excellent option for researchers who need to purify multiple exosome samples.

To isolate exosomes from tissue culture media, simply:

  • Add an appropriate volume of ExoQuick-TC
  • Incubate overnight at 4°C
  • Isolate exosomes with a 30-minute low-speed spin (1500g).

Isolated exosomes can be found in the pellet and resuspended in an appropriate solution.

With ExoQuick-TC, you can obtain high-quality exosomes from tissue culture media and certain biofluids using a protocol that can easily be performed on multiple samples and requires very low volumes of input sample