The DNA Pure Kit was designed to purify or concentrate DNA products which have been previously isolated using a variety of isolation methods. The unique DNA Pure Buffer ensures easy binding of DNA to the glass fiber matrix of the spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the ultra pure DNA is eluted by a low salt Elution Buffer, TE or water. Salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation. For ultra pure RNA, the RNA Pure Kit is also available.
Advantages (Cat. # DP100, DP300)
Recover up to 90% of DNA products within 10 minutes!
Sample Volume: up to 100 μl of DNA products
DNA glass fiber spin columns (blue membrane optimized for purifying DNA products)
Storage: room temperature for up to 2 years
Applications
PCR, Fluorescent or Radioactive Sequencing, Restriction Enzyme Digestion, DNA Labeling, Ligation
Components
DNA Pure Buffer
Wash Buffer
Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
DP Columns
2 ml Collection Tubes
Quality Control
The quality of the DNA Pure Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. DNA of various sizes are purified from aqueous solutions and analyzed by electrophoresis.
