Recreating the natural cell niche is key to successful cultivation. The physical, topological, and biochemical expression of the different laminin isoforms in the BM is heterogeneous and tissue-specific.
Biolaminin 521 MX (MX521) is the natural laminin for pluripotent stem cells and therefore reliably facilitates self-renewal of human ES and iPS cells in a chemically defined, feeder-free and animal component-free stem cell culture system. MX521 enables efficient single-cell passaging of genetically stable and pluripotent stem cells without the need for any apoptosis inhibitors for the superior quality of your cells and studies. After birth, α5-laminins represent the most common laminins in the body and MX521 supports many diverse tissue cell types, such as cells from pancreas, vascular, nervous and muscular systems.
A BIOLOGICALLY RELEVANT CULTURE ENVIRONMENT
Biolaminin 521 MX is a full-length, human, recombinant laminin 521 substrate, the only one of its kind on the market, providing an optimal environment for feeder-free culture of hPSCs. Laminin 521 is a key cell adhesion protein of the natural stem cell niche, expressed and secreted by hPSCs in the inner cell mass of the embryo. The Biolaminin 521 substrate recreates a biologically relevant hPSC milieu in vitro, promoting attachment, high survival, and robust long-term self-renewal of hPSCs. In addition, the more authentic culture environment primes the pluripotent stem cells for more efficient differentiation, enhanced cell maturation, polarization and organization of specialized cell types. The laminin 521 cell culture substrates have a positive effect on stabilizing and homogenizing pluripotent gene expression profiles between hES cell lines, providing a more controllable and robust culture system compared to the feeder- and many other feeder-free culture systems. Via the interaction with relevant cell surface receptors, laminins activate cell signaling cascades (e.g. PI3K/Akt pathway), giving consistent cellular responses and improved cell functionality, ultimately saving researchers both time and cost.
The MX521 stem cell substrate enables high expansion rate, monolayer homogenous growth, and maintained pluripotency. Cells are genetically stable without karyotypic abnormalities. hESC and iPSC grow faster on MX521 compared to cells cultured on feeders or on another feeder-free matrice, facilitating automation. Human pluripotent stem cells can routinely be cultured as single cells without the addition of ROCKi. MX521 also supports efficient clonal survival and expansion of hES and iPS cells, greatly facilitating the study of genomic alterations and manipulations in pluripotent cells. Furthermore, it renders more efficient reprogramming of specific tissue cells to iPSCs.
The Biolaminin 521 substrate is more flexible, easier to use and less laborious compared to feeder-based culture systems and is compliant with any commercially available serum-free medium. The cells grow in a homogeneous monolayer and are easy to monitor, with maintained pluripotency and genetic integrity. The robust and flexible culture environment allows operator-independent culture maintenance and reliable, standardized protocols, which can easily be adapted to automation platforms.
After birth, laminin 521 represents one of the most common laminins in the body and together with the other laminin isoforms, it supports efficient expansion and differentiation of many diverse tissue cell types.
