Description

Description:
Based on our innovative and proprietary lipid-conjugation technology, LipoJet™ Transfection Kit, formulated from novel fluorinated cationic lipids, exhibits significant difference from other lipids transfection reagents in the market. LipoJet™ Transfection Kit is the most powerful yet very gentle gene delivery tool for a variety of applications including plasmid DNA and/or siRNA for most of mammalian cell types. Compared with leading products in the market, LipoJet™ is more cost-effective and always provides higher transfection efficiency with less cytotoxicity.

Kit content:
- LipoJet™ Reagent, 1.0 ml sufficient for 1000 DNA transfections and 1000 siRNA transfections (24-well plate).
- LipoJet™ Transfection Buffer (5x ), 8.0 ml to make 40 ml of working solution (1x ).

Features:
- Versatile: DNA transfection, siRNA transfection and DNA/siRNA co-transfection.
- Powerful: Outstanding efficiency on adherent cells.
- Extremely low toxicity: Improve Cell viability after transfection
- Economical: More transfections with less reagent
- Easy to use: One tube reaction and compatible with serum/antibiotics.
- Suitable for high-throughput application

Broad Transfection Spectrum for Mammalian Cell Types

Cell Line	DNA Transfection	siRNA Transfection
293, 293T 3T3, NIH 3T3 3T3-L1 A549 B16-F10 BNLCL.2 C2C12 C3H/10T1/2 Caco-2 Rat primary cardiomyocytes CHO COS1 COS7 Human bladder carcinomal cell mES iMEF HBEC HCT116 Hela HepG2 HT-29 HuH-7 LNCaP MC3T3-E1 MCF10A MCF7 MDA-MB-231 MDCK MEF Human primary melanocytes Human primary melanoma Human immortalized microglial mIMCD-3 MRC-5 N2A PC3 RAW 264.7 RPE SH-SY-5Y SK-OV-3 U-2OS VERO	85% 65% 30% 80% 75% 55% 80% 60% 20% 10% 80% 70% 75% 80% 50% 55% 45% 75% 80% 60% 45% 45% 45% 50% 40% 50% 50% 20% 50% 50% 50% 50% 50% 50% 68% 70% 45% 30% 75% 70% 80% 40%	- 90% at 20 nM - 90% at 10 nM - - 90% at 10 nM - 80 % at 20 nM - - - - - - - - - - - - - - - - - - - 85% at 30 nM 85% at 30 nM - - - - - - - - 90% at 25 nM - - -

Examples Showing Excellent DNA and siRNA Transfection Efficiency of LipoJet™ In Vitro Transfection Kit
LipoJet Transfection Reagent
A efficiency comparison of LipoJet™ reagent vs. brand name products to transfect Hela, Cos-7, NIH-3T3, CHO and HEK293 (left panel) and Cos-7 (right panel). pEGFP-N3 cDNA (0.5 µg/well of 24-well plate) was transfected into different mammalian cells per the standard transfection protocols in presence of serum (10% FBS) and antibiotics as recommended by manufacturers. The transfection efficiency were analyzed via FACS 48 hours post transfection.

LipoJet_Cell_Viability
A toxicity comparison of LipoJet™ reagent vs. brand name products to transfect Hela cells. pEGFP-N3 cDNA (1.0 µg/well of 24-well plate) was transfected to Hela cells per the standard transfection protocols in presence of serum (10% FBS) and antibiotics as recommended by manufacturers. The MTT assay (right panel) and phase contrast imaging (left panel) were used to analyze the cell viability 48 hours post transfection.

LipoJet_LIPO2000_POLYJET_Comparison_HEK293
A comparison of LipoJet™ reagent vs. Lipofectamine 2000 (L2K) and PolyJet™ transfection reagent on HEK293T cell. pEGFP-N3 cDNA (0.125 µg/well, 0.25 µg/well and 0.5 µg/per well of 24-well plate) was transfected into 293T cells using the standard transfection protocols in presence of serum (10% FBS) with LipoJet™ (upper panel at LipoJet™/DNA (µl/µg) ratio=2), L2K (middle panel at L2K/DNA (µl/µg) ratio=3) and PolyJet™ (lower panel at at PolyJet™/DNA (µl/µg) ratio=3) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 48 hours post transfection.

LipoJet_LIPO2000_POLYJET_Comparison_HELA
A comparison of LipoJet™ reagent vs. Lipofectamine 2000 (L2K) and PolyJet™ transfection reagent on Hela cell. pEGFP-N3 cDNA (0.125 µg/well, 0.25 µg/well and 0.5 µg/per well of 24-well plate) was transfected into Hela cells using the standard transfection protocols in presence of serum (10% FBS) with LipoJet™ (upper panel at LipoJet™/DNA (µl/µg) ratio=2), L2K (middle panel at L2K/DNA (µl/µg) ratio=3) and PolyJet™ (lower panel at at PolyJet™/DNA (µl/µg) ratio=3) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 48 hours post transfection.

LipoJet_GFP_siRNA_HEK293
LipoJet_GFP_siRNA_HELA
LipoJet_GFP_siRNA_SaoS-2
Exceptional gene silencing of LipoJet™ reagent during DNA/siRNA co-transfection on mammalian cells. Transfection of GFP cDNA (left panel, 0.25 µg per well of 24-well plate) and co-transfection of GFP cDNA ( 0.25 µg per well of 24-well plate) and GFP targeted siRNA (final 10 nM, right panel) with LipoJet™ reagent leads to exceptional gene silencing on HEK293 (upper panel), Hela (middle panel) and SaoS-2 (lower panel) cells respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 48 hours post transfection.

LipoJet_HUVEC_mCherry-FITC-siRNA
Exceptional DNA/siRNA co-transfection efficiency on HUVEC. Co-transfection of mCherry cDNA (0.10 µg per well of 24-well plate) and FITC conjugated siRNA (final 30 nM per well of 24-well plate) to HUVEC with LipoJet™ reagent gave rise to 60% mCherry+ (phase contrast overlapped with mCherry imaging, left panel) and nearly 100% FITC-siRNA+ (phase contrast overlapped with FITC imaging, right panel) HUVEC 24 hours after transfection. The pictures were given from Dr. Pan Kong of USC as courtesy.

LipoJet_EG5_HEK293
LipoJet_EG5_HELA
Excellent silencing of endogenously expressed KIF11 (also known as EG5) in HEK293 (upper panel) and Hela (lower panel) cells with LipoJet™ reagent at 10 nM EG5 siRNA. KIF11 (also known as EG5) encodes a motor protein that belongs to the kinesin-like protein family involved in chromosome positioning and bipolar spindle formation during cell mitosis. A reduction in KIF11 levels causes mitotic arrest. LipoJet™ reagent effectively delivers EG5 siRNA (final 10 nM) to HEK293 and Hela cells, leading to more than 80% of "round-up" phenotype of HEK293 and Hela cells 24h post transfection over negative control (final 10 nM with sham EG5 siRNA). The phenotype of "rounded-up" HEK293 and Hela cells were visualized 24h post transfection with a Nikon microscope.