BLUltra Prestained Protein Ladder
10 prestained proteins covering 6.5 to 270 kDa, ready to use
SKU
PM001-0500
Description
The BLUltra Prestained Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 6.5 to 270 kDa. Proteins are covalently coupled with a blue chromophore except for three reference bands (two orange bands at 30 kDa and 270 kDa and one green band at 52 kDa) when separated on SDS-PAGE (Tris-glycine buffer).
The BLUltra Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in the gel loading buffer and is ready to use.
Special Features:
- Wide range: 6.5 to 270 kDa
- Reference Bands: Two orange at 30 kDa & 270 kDa, one green at 52 kDa
- Ready to Use: supplied in loading buffer for direct loading on gels
- Clear Bands: 10 sharp bands in three color protein standard for easy visualization
- Quality control: tested on lot-to-lot basis to ensure consistent product quality
Related Products
Specifications
| Product Name: | BLUltra Prestained Protein Ladder | Catalog Number: | PM009-0500 |
| Molecular Weight Range: | 6.5 to 270 kDa | Reference Bands: | 10 |
| Color: | 3-color (Blue,Green,Red) | Package: | 500μl x 1 vial |
| Prestained: | Yes | Ready To Use: | Yes |
| Loading Buffer: | 62.5 mM Tris-H3PO4 (pH 7.5 at 25 °C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3, 33% glycerol |
Recommended Loading Volume: | 3µl per well (15well) for electrophoresis 5µl per well (10well) for electrophoresis 2.5µl per well for western blotting |
| Number Of Applications: | Sufficient for 100 application (5μl per loading) Sufficient for 165 application (3μl per loading) |
Storage: | Stable for up to 2 weeks at 25°C Stable for up to 3 months at 4°C Stable for up to 24 months at -20°C |
BLUltra (PM001-0500) Safety Data SheetVideo
- One-Vial-Fits-All: New broad-range protein ladder from 6.5kDa to 270kDa.
- Higher Added Value: Tri-colors and 10 bands with premium quality.
- Better performance for higher molecular weight proteins.
Clear separation map from 6.5 kDa to 270 kDa in the Tris-Glycine system. - Enhanced transfer, wash, and stripping durabilities.
In lane 7: BLUltra Prestained Protein Ladder (PM001-0500) 5µl
NuPAGE Bis-Tris 4-12% was used to run SDS-PAGE in MOPS and MES buffer. 5ul of protein ladder samples was loaded into each lane.
Figures
Migration patterns of BLUltra Prestained Protein Ladder in different electrophoresis conditions
Note: The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards; supplemental data should be considered for more accurate adjustment in different electrophoresis conditions.
Under suggested conditions, BLUltra Prestained Protein Ladder resolves 10 major bands in 4-12% Bis-Tris Gel(MES buffer) and after Western blotting to the nitrocellulose membrane.
- 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
- 2.5 μl per well for general Western transferring.
All products are for research use only.
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Transfer your target proteins and protein ladder to a Western Blot membrane for detection with our chemiluminescent substrates
FAQs
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This is stable up to 24 months at -20°C
Yes, BlueUltra has a range from 6.5 to 270 kDa
BlueUltra has a fragment at 270kDa
No, this protein ladder is ready to use
No, this protein ladder is ready to use
yes, protein marker is another name for protein ladder same as protein molecular weight marker, protein MW marker and protein standards