12 prestained proteins covering 11 to 245 kDa, ready to use
Starting at $129.00
The BLUeye Prestained Protein Ladder is a three-color protein standard with 12 pre-stained proteins covering a wide range molecular weights from 11 to 245 kDa.
The 12 recombinant proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).
The BLUeye Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use.
Special Features:
| Product Name: | BLUeye Prestained Protein Ladder | Catalog Number: | PM007-0500 |
| Molecular Weight Range: | 11 to 245 kDa | Reference Bands: | 12 |
| Color: | 3-color (Blue,Green,Red) | Protein Concentration: | Approximately 0.1~0.4 mg/ml of each protein |
| Prestained: | Yes | Ready To Use: | Yes |
| Recommended Gel: | SDS-PAGE (Tris-glycine buffer) | Package: | 500μl x 1 vial |
| Loading Buffer: | 20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol |
Recommended Loading Volume: | 3µl per well (15well) for electrophoresis 5µl per well (10well) for electrophoresis 1.5 - 2.5µl per well for western blotting |
| Number Of Applications: | Sufficient for 100 application (5μl per loading) Sufficient for 165 application (3μl per loading) |
Storage: | Stable for up to 2 weeks at 25°C Stable for up to 3 months at 4°C For long term storage,store at -20°C |
In Lane 3: BLUeye prestained protein ladder (PM007-R500)
NuPAGE Bis-Tris 4-12% was used to run SDS-PAGE in MOPS and MES buffer. 5µl of protein ladder samples were loaded into each lane.
Gunn, S. A., Kreps, L. M., Zhao, H., Landon, K., Ilacqua, J. S., & Addison, C. L. (2022). Focal adhesion kinase inhibitors prevent osteoblast mineralization in part due to suppression of Akt-mediated stabilization of osterix. Journal of Bone Oncology, 100432.
Article | PM007-0500
Allen, V., Coulombe, J., Zhao, H., Kreps, L. M., Cook, D. P., Pryce, B., ... & Addison, C. L. (2022). VIVA1: a more invasive subclone of MDA-MB-134VI invasive lobular carcinoma cells with increased metastatic potential in xenograft models. British Journal of Cancer, 1-13.
Article | PM007-0500
van Ballegooie, C., Man, A., Pallaoro, A., Bally, M., Gates, B. D., & Yapp, D. T. (2021). Gold–Protein Composite Nanoparticles for Enhanced X-ray Interactions: A Potential Formulation for Triggered Release. Pharmaceutics, 13(9), 1407.
Article | PM007-0500
Szendrey, J., Lamothe, S. M., Vanner, S., Guo, J., Yang, T., Li, W., ... & Zhang, S. (2019). Anti-Ro52 antibody acts on the S5-pore linker of hERG to chronically reduce channel expression. Cardiovascular research, 115(10), 1500-1511.
Article | PM007-0500
Szendrey, J., Lamothe, S. M., Vanner, S., Guo, J., Yang, T., Li, W., ... & Zhang, S. (2019). Anti-Ro52 antibody acts on the S5-pore linker of hERG to chronically reduce channel expression. Cardiovascular research, 115(10), 1500-1511.
Article | PM007-0500
Migration patterns of BLUeye Prestained Protein Ladder in various electrophoresis conditions:
• 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
• 1.5~2.5 μl per well for general Western transferring.
Under suggested conditions, BLUeye Prestained Protein Ladder resolves 12 bands in 4-15% SDS-PAGE (Trisglycine buffer) and after Western blotting to a nitrocellulose membrane.Apply more for thicker (> 1.5 mm) or larger gel.
Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.
Transfer your target proteins and protein ladder to a Western Blot membrane for detection with our chemiluminescent substrates .
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BLUelf has an additional band at 5bp
3-5µl per well
Store at -20°C for long term stability
No, Froggabio protein standards are ready to load. We do not recommend heating/boiling them as this may cause degradation of proteins in the standard
No, this protein ladder is ready to use
No, this protein ladder is ready to use
yes, protein marker is another name for protein ladder same as protein molecular weight marker, protein MW marker and protein standards
