Skip to the beginning of the images gallery

BLUelf Prestained Protein Ladder

BLUeye Prestained Protein ladder

BLUelf Prestained Protein ladder

Be the first to review this product

13 prestained proteins covering 5 to 245 kDa, ready to use

$129.00

SKU

PM008-0500

Size: 500μl

Buy 8 for $62.38 each and save 52%

Description

Related Products

Specifications

Protocol

Video

Figures

FAQs

Description

The BLUelf Prestained Protein Ladder is a three-color protein standard with 13 pre-stained proteins covering a wide range molecular weights from 5 to 245 kDa.Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).
The BLUelf Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The protein ladder is supplied in gel loading buffer and is ready to use.

Special Features:

Wide range: 5 to 245 kDa
Reference Bands: One Green at 25 kDa, one Red at 75 kDa
Ready to Use: Supplied in loading buffer for direct loading on gels
Clear Bands: 13 sharp bands in three color protein standard for easy visualization
Quality control: Tested on lot-to-lot basis to ensure consistent product quality

Related Products

Specifications

Product Name:	Bluelf Prestained Protein Ladder	Catalog Number:	PM008-0500
Molecular Weight Range:	5 to 245 kDa	Reference Bands:	13
Color:	3-color(Blue,Green,Red)	Protein Concentration:	Approximately 0.1~0.4 mg/ml of each protein
Prestained:	Yes	Ready To Use:	Yes
Recommended Gel:	SDS-PAGE (Tris-glycine buffer)	Package:	500μl x 1 vial
Loading Buffer:	20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol	Recommended Loading Volume:	3µl per well (15well) for electrophoresis 5µl per well (10well) for electrophoresis 2.5µl per well for western blotting
Number Of Applications:	Sufficient for 100 application (5μl per loading) Sufficient for 165 application (3μl per loading)	Storage:	Stable for up to 2 weeks at 25°C Stable for up to 3 months at 4°C Stable for up to 24 months at -20°C

Click to compare and view all other protein ladders

Protocol

BLUelf (PM008-0500) Safety Data Sheet

BLUelf (PM008-0500) Protocol

Video

In Lane 2: BLUelf prestained protein ladder (PM008-R500) 5µl

NuPAGE Bis-Tris 4-12% was used to run SDS-PAGE in MOPS and MES buffer. 5µl of protein ladder samples were loaded into each lane.

Figures

Migration patterns of BLUelf Prestained Protein Ladder in various electrophoresis conditions:

refers to bluelf prestained protein ladder patterns in various electrophoresis conditions

Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.

3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively
2.5 μl per well for general Western transferring
Apply more for thicker (> 1.5 mm) or larger gel

All products are for research use only.

More To Explore:

Transfer your target proteins and protein ladder to a Western Blot membrane for detection with our chemiluminescent substrates .

FAQs

Got a question? We're here to answer! If you don't see your question here, drop us a line on our Contact Us page.

Three, this is a tri-coloured protein ladder

3-5μl per well

PVDF, nylon, and nitrocellulose

No, this protein ladder is ready to use

No, Froggabio protein ladders are ready to load. We do not recommend heating/boiling them as this may cause degradation of proteins in the standard

No, this protein ladder is ready to use

yes, protein marker is another name for protein ladder same as protein molecular weight marker, protein MW marker and protein standards