BLUelf Prestained Protein ladder
This product can be purchased in the USA exclusively through Gemini-Bio Buy Now
$129.00
SKU
PM008-0500
Description
The BLUelf Prestained Protein Ladder is a three-color protein standard with 13 pre-stained proteins covering a wide range molecular weights from 5 to 245 kDa.Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).
The BLUelf Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The protein ladder is supplied in gel loading buffer and is ready to use.
Special Features:
- Wide range: 5 to 245 kDa
- Reference Bands: One Green at 25 kDa, one Red at 75 kDa
- Ready to Use: Supplied in loading buffer for direct loading on gels
- Clear Bands: 13 sharp bands in three color protein standard for easy visualization
- Quality control: Tested on lot-to-lot basis to ensure consistent product quality
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Specifications
| Product Name: | Bluelf Prestained Protein Ladder | Catalog Number: | PM008-0500 |
| Molecular Weight Range: | 5 to 245 kDa | Reference Bands: | 13 |
| Color: | 3-color(Blue,Green,Red) | Protein Concentration: | Approximately 0.1~0.4 mg/ml of each protein |
| Prestained: | Yes | Ready To Use: | Yes |
| Recommended Gel: | SDS-PAGE (Tris-glycine buffer) | Package: | 500μl x 1 vial |
| Loading Buffer: | 20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol |
Recommended Loading Volume: | 3µl per well (15well) for electrophoresis 5µl per well (10well) for electrophoresis 2.5µl per well for western blotting |
| Number Of Applications: | Sufficient for 100 application (5μl per loading) Sufficient for 165 application (3μl per loading) |
Storage: | Stable for up to 2 weeks at 25°C Stable for up to 3 months at 4°C Stable for up to 24 months at -20°C |
Video
In Lane 2: BLUelf prestained protein ladder (PM008-R500) 5µl
NuPAGE Bis-Tris 4-12% was used to run SDS-PAGE in MOPS and MES buffer. 5µl of protein ladder samples were loaded into each lane.
Figures
Migration patterns of BLUelf Prestained Protein Ladder in various electrophoresis conditions:
Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.
- 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively
- 2.5 μl per well for general Western transferring
- Apply more for thicker (> 1.5 mm) or larger gel
All products are for research use only.
More To Explore:
Transfer your target proteins and protein ladder to a Western Blot membrane for detection with our chemiluminescent substrates .
FAQs
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Three, this is a tri-coloured protein ladder
3-5μl per well
PVDF, nylon, and nitrocellulose
No, this protein ladder is ready to use
No, Froggabio protein ladders are ready to load. We do not recommend heating/boiling them as this may cause degradation of proteins in the standard
No, this protein ladder is ready to use
yes, protein marker is another name for protein ladder same as protein molecular weight marker, protein MW marker and protein standards