13 prestained proteins covering 5 to 245 kDa, ready to use
Starting at $129.00
The BLUelf Prestained Protein Ladder is a three-color protein standard with 13 pre-stained proteins covering a wide range molecular weights from 5 to 245 kDa.Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).
The BLUelf Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The protein ladder is supplied in gel loading buffer and is ready to use.
Special Features:
| Product Name: | Bluelf Prestained Protein Ladder | Catalog Number: | PM008-0500 |
| Molecular Weight Range: | 5 to 245 kDa | Reference Bands: | 13 |
| Color: | 3-color(Blue,Green,Red) | Protein Concentration: | Approximately 0.1~0.4 mg/ml of each protein |
| Prestained: | Yes | Ready To Use: | Yes |
| Recommended Gel: | SDS-PAGE (Tris-glycine buffer) | Package: | 500μl x 1 vial |
| Loading Buffer: | 20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol |
Recommended Loading Volume: | 3µl per well (15well) for electrophoresis 5µl per well (10well) for electrophoresis 2.5µl per well for western blotting |
| Number Of Applications: | Sufficient for 100 application (5μl per loading) Sufficient for 165 application (3μl per loading) |
Storage: | Stable for up to 2 weeks at 25°C Stable for up to 3 months at 4°C Stable for up to 24 months at -20°C |
In Lane 2: BLUelf prestained protein ladder (PM008-R500) 5µl
NuPAGE Bis-Tris 4-12% was used to run SDS-PAGE in MOPS and MES buffer. 5µl of protein ladder samples were loaded into each lane.
Alston, B. M., Rainwater, T. R., Parrott, B. B., Wilkinson, P. M., Bowden, J. A., & Rice, C. D. (2022). Quantifying Circulating IgY Antibody Responses against Select Opportunistic Bacterial Pathogens and Correlations with Body Condition Factors in Wild American Alligators, Alligator mississippiensis. Biology, 11(2), 269.
Article | PM008-0500
Boudreault, S., Durand, M., Martineau, C. A., Perreault, J. P., Lemay, G., & Bisaillon, M. (2022). Reovirus μ2 protein modulates host cell alternative splicing by reducing protein levels of U5 snRNP core components. Nucleic acids research, 50(9), 5263-5281.
Article | PM008-0500
Bel, J. S., Tai, T. C., Khaper, N., & Lees, S. J. (2022). Chronic glucocorticoid exposure causes brown adipose tissue whitening, alters whole‐body glucose metabolism and increases tissue uncoupling protein‐1. Physiological Reports, 10(9), e15292.
Article | PM008-0500
von Aderkas, P., Little, S., Nepi, M., Guarnieri, M., Antony, M., & Takaso, T. (2022). Composition of Sexual Fluids in Cycas revoluta Ovules During Pollination and Fertilization. The Botanical Review, 1-32.
Article | PM008-0500
Sivaranjani, M., Hansen, E. G., Perera, S. R., Flores, P. A., Tükel, Ç., & White, A. P. (2022). Purification of the Bacterial Amyloid “Curli” from Salmonella enterica Serovar Typhimurium and Detection of Curli from Infected Host Tissues. Bio-protocol, 12(10), e4419-e4419.
Article | TM300-0.22PVDF | PM008-0500
Migration patterns of BLUelf Prestained Protein Ladder in various electrophoresis conditions:
Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.
All products are for research use only.
Transfer your target proteins and protein ladder to a Western Blot membrane for detection with our chemiluminescent substrates .
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Three, this is a tri-coloured protein ladder
3-5μl per well
PVDF, nylon, and nitrocellulose
No, this protein ladder is ready to use
No, Froggabio protein ladders are ready to load. We do not recommend heating/boiling them as this may cause degradation of proteins in the standard
No, this protein ladder is ready to use
yes, protein marker is another name for protein ladder same as protein molecular weight marker, protein MW marker and protein standards
