AzuraQuant cDNA Synthesis Kit provides a rapid and sensitive method for generating high quality, qPCR-ready first-strand cDNA for use in downstream real-time PCR.
AzuraQuant cDNA Synthesis Kit provides a rapid and sensitive method for generating high quality, qPCR-ready first-strand cDNA for use in downstream real-time PCR. The AzuraQuant cDNA Synthesis Kit exhibits highly reproducible, unbiased synthesis and representation of 5' and 3' mRNA ends. The Kit is supplied in a convenient, two tube format comprised of a 5x Buffer and 20x HighTherm reverse transcriptase blended with RNase inhibitor. The kit was developed to provide consistent linearity across a wide range of input RNA, revealing accurate relative cDNA representation, regardless of gene abundance. HighTherm reverse transcriptase provides exceptional thermostability and is blended with a potent RNase inhibitor to ensure high integrity of Total RNA starting material. The AzuraQuant cDNA Synthesis Kit delivers both highly efficient first-strand synthesis and higher cDNA yields, leading to enhanced reproducibility and data accuracy, and provides an ideal chemistry for success with limited starting material, such as tissue biopsies.
