TruFi II Ultra 2x PCR Red Mix is an optimized 2x master-mix comprised of TruFi II DNA Polymerase, optimized reaction buffer, MgCl2, ultra-pure dNTPs, and an inert red gel-loading dye.
TruFi II Ultra 2x PCR Red Mix is an optimized 2x master-mix comprised of TruFi II DNA Polymerase, optimized reaction buffer, MgCl2, ultra-pure dNTPs, and an inert red gel-loading dye. This red gel loading and tracking dye simplifies downstream gel analysis by allowing samples to be loaded directly in agarose gel wells. The red dye front migrates at the rate of 1000 bp - 2000 bp DNA fragments in 0.5 percent to 1.5 percent agarose gels. TruFi II Ultra 2x PCR Red Mix is ideal for fast cycling, as the polymerase exhibits a rapid elongation rate of 10 seconds per kb. The ready-to-use 2x mix is also highly efficient in long PCR with amplicons greater than 15 kb. With an error rate which is greater than 65x lower than Taq Polymerase, TruFi II Ultra 2x PCR Mix is recommended for applications which require extremely high fidelity such as cloning, NGS applications, and mutagenesis.
