TruFi II Ultra 2x PCR Mix is an optimized 2x master-mix comprised of TruFi II DNA Polymerase, optimized reaction buffer, MgCl2, and ultra-pure dNTPs. TruFi II Ultra 2x PCR Mix is ideal for fast cycling, as the polymerase exhibits a rapid elongation rate of 10 seconds per kb. The ready-to-use 2x mix is also highly efficient in long PCR with amplicons greater than 15 kb. With an error rate which is greater than 65x lower than Taq Polymerase, TruFi II Ultra 2x PCR Mix is recommended for applications which require extremely high fidelity such as cloning, NGS applications, and mutagenesis.