ExtremeTaq HiFi Mix is an optimized 2x master-mix comprised of an enhanced Taq DNA Polymerase, optimized reaction buffer, MgCl2, and ultra-pure dNTPs; ideally suited to all routine end-point PCR applications and challenging DNA targets such as complex GC-rich DNA and low-copy number samples.
The Universal All-in-One Mix for Everyday PCR: ExtremeTaq HiFi Mix is an optimized 2x master-mix comprised of an enhanced Taq DNA Polymerase, optimized reaction buffer, MgCl2, and ultra-pure dNTPs; this versatile master mix is ideally suited to all routine end-point PCR applications and challenging DNA targets such as complex GC-rich DNA and low-copy number samples. The formulation contains Taq Polymerase, proprietary enhancers, hot-start antibodies, and a proof-reading component for trouble-free PCR reaction assembly and consistent performance. ExtremeTaq HiFi Mix delivers a unique balance of PCR sensitivity, high fidelity, and value. The ready-to-use ExtremeTaq HiFi Mix provides excellent performance with plasmid DNA, cDNA and complex mammalian DNA, and exhibits up to 10x higher fidelity than Taq polymerase. The highly efficient buffer and hot-start blend provide the ideal conditions for high-performance PCR and inactivity at room temperature, thereby eliminating non-specific amplification.