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Aurox Unity and Clarity - Spinning Disk Confocal

Aurox Unity Life

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Complete confocal microscope for live cell imaging.

True to its name, Unity^life brings together in one fully integrated, compact and easy to use microscope, everything you need for confocal imaging on your bench top.

With an integrated stage-top incubator and connection for CO₂, Unity^life is the ideal confocal live cell imaging system.

SKU

DS00100

Size: 1 unit

Description

Specifications

Application Image Gallery

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Description

All-in-one bench-top High resolution Inverted microscope.

Laser Free Confocal

Designed around Aurox’s laser-free confocal technology, unity combines Aurox’s patented structured illumination spinning disc with state-of-the-art technology and a unique, novel optical arrangement. The results are high quality confocal images recorded quickly and easily in your laboratory.

Structured Illumination Spinning Disk

Aurox' laser free spinning disc uses a grid pattern which achieves high light efficiency with a speed of a spinning disc. This allows the use of laser free light sources, giving a high quality confocal images.

Unity brings together in one fully integrated, compact and easy to use microscope, everything you need for live-cell confocal imaging.

Stage incubator & Co2 connections

Unity’s red cased incubator sample stage provides heating and CO2 gas connections for sample environment control, allowing your live-cell samples to be maintained at 35-37 degrees Celsius and under CO2 for the duration of your longer term imaging experiments.

Fast multichannel acquisition

Red, blue, green - set 1, 2 or 3 colour channels at a time. One tap each switches on or off the colour channels. Set exposure times and brightness by dragging up or down on the respective colour chevrons. Handy histograms help you achieve optimal settings.

Easy to use- Plug & Play

Gone are the tangle of cables found on other confocal systems. Unity requires a single power cable and has rear ports for the connection of a CO2 supply. Unity can be networked wirelessly, but it also has a wired network connection and multiple USB-3 / USB-c ports if required.

Compact- Fits on lab bench

Designed for the laboratory bench-top, Unity is extremely compact and self-contained, weighing less than 25 Kg and has an integrated vibration dampening.

Unity is ideal for use in the light and airy open laboratory,

Easy to use with minimal interaction
Easy to move and locate
Easy to maintain / clean
Plug and play

For slides, plates and dishes

Unity is supplied with three interchangeable sample holders allowing you to image all your samples, whether they are mounted in multi-well plates, petri-dishes or slides. Each of the Unity sample holders has a retention system to hold the sample securely in the holder and a three point magnetic location system which connects to Unity's XY stage on loading. This system ensures the sample holder position is securely held throughout imaging experiments

Specifications

Confocality:	0.6 μm (FWHM) with 1.4 N.A oil objective
Disc:	1 sector structured illumination disc.
Minimum exposure:	20 ms
Maximum frame rate:	Up to 40 fps (1 colour) Up to 13 fps (3-colours)
Imaging channels:	3 integrated filter channels, DAPI, FITC, TRITC.
Channel switching:	Instantaneous.
Detector:	Integrated sCMOS 2x (2048 x 2048), 6.5 µm pixels.
Excitation range:	370 - 700 nm
Emission range:	410 - 750 nm

Application Image Gallery

Daphnia tilescan

Tile scan Z-projection image of Daphnia autofluorescence. Imaged with Aurox Unity and 20 x 0.75 NA, Water immersion objective. FITC (20 ms exposure) /DsRED (20 ms exposure) excitation channels used for imaging. 128 slices, 1 micron steps, 9 tiles. Total time of acquisition = 102 seconds. Prepared Slide from Brunel Microscopes Ltd

Mouse kidney tilescan

Tile scan of mouse kidney recorded on Unity over 4 mins. FluoCells Prepared Slide #3 (mouse kidney section with Alexa Fluor 488 WGA, Alexa Fluor 568 Phalloidin, and DAPI). 60x 1.4 NA Oil (DAPI 20ms exposure, FITC 20ms exposure, TRITC 20ms exposure), 75 slices, 0.3 micron steps, 20 tiles.

Zebrafish tilescan

Tile of 3x1 images each a Z-projection image of 70 frame Z-stack over a 210µm range. Unity was fitted with a 10x 0.45NA lens. Green is myoII staining. 3x 70 images of 40 msec exposure (FITC) and 100ms (DsRED) per image. Time of acquisition was 13.3 secs per tile giving a total time for acquistion of 40 secs. Each tile was 1200 x 1200 µm. DAPI, FITC and DsRed LED Tri-band filter cube used. Samples courtesy of Dr Florencia Cavodeass, St. George's University of London.

THP-1 macrophages

THP-1 cells differentiated with PMA towards a macrophage phenotype. Video shows phagocytosis of bacteria. Imaged with Aurox Unity and 60x 1.4 NA, dry objective. Tubulin labelled with AF555, bacteria labelled with AF488, nucleus labelled with DAPI. Video prepared using Andor Imaris software. Sample courtesy of Riccardo Wysoczanski, Heart and Lung Institute, Imperial College London.

Mosquito heads ( male and female comparison ) tilescan

Tile scan Z-projection image of male and female autofluorescence mosquito heads. Imaged with Aurox Unity and 10 x 0.45 NA, dry objective. DAPI (80 ms exposure) FITC (80 ms exposure, but not shown in final image) /DsRED (300 ms exposure) excitation channels used for imaging. 94 slices, 3 micron steps, 12 tiles. Total time of acquisition = 147 seconds. Prepared Slide from Brunel Microscopes Ltd

Dictydium Slime Mould tilescan

Tilescan image of Dictydium slime mould recorded on Unity using a 10x, 0.45 NA dry objective. 4 x 2 tilescan red autofluorescence. 111 slices, 3 micron step size. All images have been cropped from original image size and stitched using Andor Imaris. Video produced in Imaris Slime mould samples from Carolina Biological Supply, supplied by Blades Biological

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